The Application of SDS-PAGE Protein and HPTLC Amino Acid Profiling for Verification of Declared Variety and Geographical Origin of Honey

Abstract Proteins and amino acids are minor components of honey that rarely used for its quality evaluation, although these create biological activity as well can serve overheating detection. The aim the study was to use indicators confirm a declared on label variety. Fifty-eight samples 8 different varieties meeting commercial requirements were used, including 28 local Polish 30 (mainly imported from EU non-EU) honeys. For protein profiling previously polyacrylamide electrophoresis in denaturing conditions (SDS-PAGE) applied whereas free acid profile analyzed by high-performance thin-layer chromatography (HPTLC) first time. As auxiliary indicators, colorimetric determination content Bradford method, 5 glycolytic enzymes, diastase, β-galactosidase, N-acetyl-β-glucosaminidase, α-mannosidase, α-glucosidase, proline used. It has been shown based determined model SDS-PAGE profiles selected monofloral honeys, it is possible detect honeys questionable variety lack specific bands or their diverging intensity. HPTLC analysis supporting control tool, capturing differences profile. Due great variation multifloral honey, such assays effective only. assays, especially total be also useful. tools proposed initial verification cost reduction officially recognized melissopalynological analysis.